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The UCI, world cycling's governing body, found that the concentration of the substance in his urine was double the permitted levels, but he is not accused of overdosing.
Froome, who thanked fans for their support after news of the probe broke this morning, has had problems with asthma all his life and has always been open about his use of an inhaler.
"I take my leadership position in my sport very seriously.
The UCI is absolutely right to examine test results and, together with the team, I will provide whatever information it requires.” Later on Wednesday evening he spoke with the BBC Sport, he said: "I understand this comes as a big shock to people.
But there are concerns use of permitted substances are being abused in endurance sports.
And he added: "The sport is coming from a very dark background and I have tried to do everything through my career to show that the sport has turned around." The UCI claimed Froome was notified on September 20 of his "adverse analytical finding" from the September 7 sample.
Then there was the infamous scandal over a "jiffy bag" that was allegedly sent to the Team Sky bus at the Criterium du Dauphine in 2011.
But he and Sky were cleared of any wrongdoing after a lengthy investigation by UK Anti-Doping - although UKAD expressed "serious concern" over the lack of proper medical records at British Cycling.
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The 32-year-old, who could have his title stripped from him, said on : "It is well known that I have asthma and I know exactly what the rules are.
Thank you for all the messages of support this morning.
But this time, his result of 2,000 nanograms of Salbutamol per ml was double the permissible limit of 1000ng/ml in a test taken on September 7.
That day, he was riding from Suances to Santo Toribio de Liebana in the red leader's jersey of La Vuelta and had only three stages to go - including the race's infamous hardest climb, the Alto de L' Angliru, two days later.